重组
RabMAb

Anti-OVCA1抗体[EPR15342] - C-terminal (ab185960)

概述

  • 产品名称
    Anti-OVCA1抗体[EPR15342] - C-terminal
    参阅全部 OVCA1 一抗
  • 描述
    兔单克隆抗体[EPR15342] to OVCA1 - C-terminal
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment within Human OVCA1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BZG8

  • 阳性对照
    • SK OV 3, HeLa, SKBR3 and A375 cell lysates.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab185960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IP 1/30 - 1/50.

靶标

  • 功能
    Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival.
  • 组织特异性
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors.
  • 序列相似性
    Belongs to the DPH1/DPH2 family. DPH1 subfamily.
  • 细胞定位
    Nucleus. Cytoplasm. Punctate, primarily perinuclear localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Candidate tumor suppressor in ovarian cancer 1 antibody
    • Diphthamide biosynthesis protein 1 antibody
    • Diphthamide biosynthesis protein 2 homolog like 1 antibody
    • Diphthamide biosynthesis protein 2 homolog-like 1 antibody
    • Diphthamide biosynthesis protein 2 like antibody
    • Diphthamide biosynthesis protein 2-like antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces) like 1 antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis like 1 antibody
    • DPH like 1 (S. cerevisiae) antibody
    • DPH like 1 antibody
    • DPH-like 1 antibody
    • DPH1 antibody
    • DPH1 homolog (S. cerevisiae) antibody
    • DPH1 homolog antibody
    • DPH1_HUMAN antibody
    • DPH2 like 1 antibody
    • DPH2-like 1 antibody
    • DPH2L antibody
    • DPH2L1 antibody
    • FLJ33211 antibody
    • HsDph1 antibody
    • Ovarian cancer associated gene 1 antibody
    • Ovarian cancer associated gene 1 protein antibody
    • Ovarian cancer-associated gene 1 protein antibody
    • Ovarian tumor suppressor candidate 1 antibody
    see all

图片

  • Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/10000 dilution

    Lane 1 : SKBR3 cell lysate
    Lane 2 : A375 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 49 kDa

  • All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/1000 dilution

    Lane 1 : SK OV 3 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa

文献

ab185960 has not yet been referenced specifically in any publications.

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