Anti-Otx2抗体(ab76748)

概述

  • 产品名称Anti-Otx2抗体
    参阅全部 Otx2 一抗
  • 描述
    兔多克隆抗体to Otx2
  • 特异性Reacts specifically with 31 kDa Otx2 protein.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from the N terminal domain of human Otx2

  • 阳性对照
    • This antibody gave a positive siganl in OTX2 Overexpression Lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab76748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200.
WB 1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).Can be blocked with Otx2 peptide (ab156847).

靶标

  • 功能Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
  • 组织特异性Expressed in brain.
  • 疾病相关Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
  • 序列相似性Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Embryo.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPHD6 antibody
    • Homeobox protein OTX2 antibody
    • MCOPS 5 antibody
    • MCOPS5 antibody
    • MGC45000 antibody
    • Orthodenticle 2 antibody
    • Orthodenticle homeobox 2 antibody
    • Orthodenticle homolog 2 (Drosophila) antibody
    • Orthodenticle homolog 2 antibody
    • Orthodenticle2 antibody
    • Otx 2 antibody
    • otx2 antibody
    • OTX2_HUMAN antibody
    see all

Anti-Otx2 antibody 图像

  • All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml

    Lane 1 : OTX1 Recombinant Protein at 0.1 µg
    Lane 2 : OTX2 Overexpression Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

Anti-Otx2 antibody (ab76748)参考文献

ab76748 has not yet been referenced specifically in any publications.

Product Wall


We have added the specific blocking peptide used to produce ab76748 to the catalog. A link for the blocking peptide is given below:
ab156847: http://www.abcam.com/otx2-peptide-ab156847.html

Our blocking protocol is given through the l...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain/Pituitary)
Specification Brain/Pituitary
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Verified customer

提交于 Jun 14 2012

Thank you for contacting us.
I'm sorry to hear that the customer has trouble with this antibody.

The information that was provided by the customer is very helpful to us and I am happy to arrange for a free of charge replacement with ab21...

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I am very pleased to hear you would like to accept our offer and test ab76748 in mouse in IHC-P. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse inIHC-Pand include th...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for getting back to me with these questions.

I have been under the assumption that you havwe been using ab21190 for your experiments, have you been using ab92515 as well?Based on sequence homology ab92515 will recognize both protei...

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Thank you for contacting us.

The immunogen for ab92515 shares 100% identity with OTX1, so cross reactivity is predicted.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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Thank you for contacting us.

I am sorry that you have found this product unsatisfactory following its recent target refinement. As we discussed on the phone the sequence for ab21990 is shared by both OTX1 and OTX2. It was because of this tha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"