概述

  • 产品名称
  • 描述
    兔多克隆抗体to Otx1
  • 特异性
    We have not been able to test Rabbit polyclonal to Otx1 (ab25985) in Western Blot due to difficulties in accessing the appropriate lysates. We would appreciate any feedback on this antibody.
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chimpanzee, Gorilla, Catfish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Otx1.

  • 阳性对照
    • This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse E11.5 Embryo Normal; Formalin Fixed Paraffin Embedded Mouse E17 Brain Normal.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
IHC-Fr 1/100.

靶标

图片

  • Anti-Otx1 antibody (ab25985) at 1 µg/ml + OTX1 (Human) - Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25985 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Ab25985 recognizes the full length tagged recombinant protein which has an expected molecular weight of 75 kDa.

  • IHC-P image of Otx1 antibody (ab25985) on E11.5 mouse embryo sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using EDTA. These sections were then blocked in serum for 1 hour, before the Otx1 antibody was added at 1:100 dilution and left overnight at 4oC.

    See Abreview

文献

This product has been referenced in:
  • Saito T  et al. Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression. Cereb Cortex : (2010). Read more (PubMed: 20624841) »
  • Hadjivassiliou G  et al. The application of cortical layer markers in the evaluation of cortical dysplasias in epilepsy. Acta Neuropathol : (2010). IHC-P ; Human . Read more (PubMed: 20411268) »

See all 2 Publications for this product

客户评价及客户问答

This antibody is guaranteed to work in human and in Immunohistochemistry on paraffinised tissue (IHC-P) so yes this antibody is suitable for use in your experiment. However, with IHC-P an enzymatic detection is usually preferable over an immunofluorese...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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