概述

  • 产品名称
  • 描述
    兔多克隆抗体to OTULIN
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human OTULIN aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: Q96BN8

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; U2OS

性能

应用

Our Abpromise guarantee covers the use of ab114137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

靶标

Anti-OTULIN antibody 图像

  • All lanes : Anti-OTULIN antibody (ab114137) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 73 kDa (possible non-specific binding).

    Exposure time : 12 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab114137 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

Anti-OTULIN antibody (ab114137)参考文献

ab114137 has not yet been referenced specifically in any publications.

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