概述

  • 产品名称
  • 描述
    兔多克隆抗体to OTUD1
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    antigen: SSAAEPVIVS RSDPRDEKLA LYLAEVEKQD KYLRQRNKYR FHIIPDGNCL YRAVSKTVYG DQSLHRELRE QTVHYIADHL DHFSPLIEGD VGEFIIAAAQ D, corresponding to internal sequence amino acids 272-372 of Human OTUD1 (Q5VV17).

  • 阳性对照
    • Human testis tissue. RT-4 cell lysate. Human plasma lysate. Human tonsil and liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab122481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-OTUD1 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122481 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122481 at 1/20 dilution staining OTUD1 in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-OTUD1 antibody (ab122481) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

Anti-OTUD1 antibody (ab122481)参考文献

This product has been referenced in:
  • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »

See 1 Publication for this product

Product Wall

Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Human Cell lysate - whole cell (HCT116, RPE-1, 293T)
Specification
HCT116, RPE-1, 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Michal Simicek

Verified customer

提交于 Sep 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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