Anti-OTUB2抗体(ab74371)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to OTUB2
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of human OTUB2.

  • 阳性对照
    • Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA 1/20000.

靶标

  • 功能
    Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
  • 组织特异性
    Widely expressed. Expressed at higher level in brain.
  • 序列相似性
    Belongs to the peptidase C65 family.
    Contains 1 OTU domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf137 antibody
    • Deubiquitinating enzyme OTUB2 antibody
    • FLJ21916 antibody
    • MGC3102 antibody
    • OTB2 antibody
    • OTU domain containing ubiquitin aldehyde binding protein 2 antibody
    • OTU domain, ubiquitin aldehyde binding 2 antibody
    • OTU domain-containing ubiquitin aldehyde-binding protein 2 antibody
    • OTU2 antibody
    • Otub2 antibody
    • OTUB2_HUMAN antibody
    • Otubain 2 antibody
    • Otubain-2 antibody
    • Ubiquitin specific processing protease OTUB2 antibody
    • Ubiquitin specific protease otubain 2 antibody
    • Ubiquitin thioesterase OTUB2 antibody
    • Ubiquitin-specific-processing protease OTUB2 antibody
    see all

图片

  • All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution

    Lane 1 : Extract from HepG2 cells
    Lane 2 : Extract from Jurkat cells
    Lane 3 : Extract from COLO 205 cells
    Lane 4 : Extract from HT-29 cells
    Lane 5 : Extract from 293 cells
    Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab74371 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carlos le Sage

Verified customer

提交于 Oct 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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