Anti-OTUB2抗体(ab74371)
Key features and details
- Rabbit polyclonal to OTUB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OTUB2抗体 -
描述
兔多克隆抗体to OTUB2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide derived from an internal region of human OTUB2.
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阳性对照
- Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74371于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
靶标
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功能
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. -
组织特异性
Widely expressed. Expressed at higher level in brain. -
序列相似性
Belongs to the peptidase C65 family.
Contains 1 OTU domain. - Information by UniProt
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数据库链接
- Entrez Gene: 78990 Human
- Entrez Gene: 68149 Mouse
- Omim: 608338 Human
- SwissProt: Q96DC9 Human
- SwissProt: Q9CQX0 Mouse
- Unigene: 278815 Human
- Unigene: 127378 Mouse
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别名
- C14orf137 antibody
- Deubiquitinating enzyme OTUB2 antibody
- FLJ21916 antibody
see all
图片
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All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution
Lane 1 : Extract from HepG2 cells
Lane 2 : Extract from Jurkat cells
Lane 3 : Extract from COLO 205 cells
Lane 4 : Extract from HT-29 cells
Lane 5 : Extract from 293 cells
Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab74371 被引用在 1 文献中.
- Zhang S et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun 9:215 (2018). PubMed: 29335437