antigen sequence: SYHITVRGRP ARISLVAQNK KQFKSRETLY GQEIDQASLL VILKLSAGDQ VWLEVSKDWN GVYVSAEDDS I, corresponding to amino acids 391-461 of Human OTOL1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the OTOL1 family.
Contains 1 C1q domain. Contains 3 collagen-like domains.
Information by UniProt
C1q and tumor necrosis factor related protein 15 antibody
Immunocytochemistry/ Immunofluorescence - Anti-OTOL1 antibody (ab122687)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and golgi apparatus. Recommended concentration of ab122687 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTOL1 antibody (ab122687)
ab122687, at 1/200 dilution, staining OTOL1 in paraffin-embedded Human colon tissue by
has not yet been referenced specifically in any publications.
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