重组
RabMAb

Anti-Osteoprotegerin抗体[EPR3592] (ab124820)

概述

  • 产品名称
    Anti-Osteoprotegerin抗体[EPR3592]
    参阅全部 Osteoprotegerin 一抗
  • 描述
    兔单克隆抗体[EPR3592] to Osteoprotegerin
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
    不适用于: Flow Cyt,IP or WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human TNFRSF11B.

  • 阳性对照
    • Fetal kidney lysate. Human kidney tissue. Human heart tissue. HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt,IP or WB.
  • 靶标

    • 功能
      Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
    • 组织特异性
      Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
    • 疾病相关
      Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.
    • 序列相似性
      Contains 2 death domains.
      Contains 4 TNFR-Cys repeats.
    • 翻译后修饰
      N-glycosylated. Contains sialic acid residues.
      The N-terminus is blocked.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MGC29565 antibody
      • OCIF antibody
      • OPG antibody
      • Osteoclastogenesis inhibitory factor antibody
      • Osteoprotegerin antibody
      • PDB5 antibody
      • TNF receptor superfamily member 11b antibody
      • TNFRSF 11B antibody
      • TNFRSF11B antibody
      • TR 1 antibody
      • TR1 antibody
      • TR11B_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 11B antibody
      see all

    图片

    • ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human heart tissue by Immunohistochemistry.
    • ab124820, at 1/100 dilution, staining TNFRSF11B in HeLa cells.

    文献

    This product has been referenced in:
    • Ramos YF  et al. A gain of function mutation in TNFRSF11B encoding osteoprotegerin causes osteoarthritis with chondrocalcinosis. Ann Rheum Dis N/A:N/A (2014). Read more (PubMed: 24743232) »

    See 1 Publication for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (MCF-7)
    Specification
    MCF-7
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample
    Human Tissue sections (Breast carcinoma)
    Specification
    Breast carcinoma
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册