Anti-Osteopontin抗体(ab8448)
Key features and details
- Rabbit polyclonal to Osteopontin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Osteopontin抗体
参阅全部 Osteopontin 一抗 -
描述
兔多克隆抗体to Osteopontin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (6) |
1/100 - 1/300.
|
ICC/IF |
1/1000.
|
说明 |
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IHC-P
1/100 - 1/300. |
ICC/IF
1/1000. |
靶标
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功能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
组织特异性
Bone. Found in plasma. -
序列相似性
Belongs to the osteopontin family. -
翻译后修饰
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
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别名
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
图片
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Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.
Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.
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ICC/IF image of ab8448 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8448, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (406)
ab8448 被引用在 406 文献中.
- Güler Ş et al. Comparison of the effectiveness of Ankaferd Blood Stopper® and Emdogain in periodontal regeneration. Oral Dis 28:1947-1957 (2022). PubMed: 33740823
- Zhu Z & Xiong J miR-141-3p Regulates EZH2 to Attenuate Porphyromonas gingivalis Lipopolysaccharide-Caused Inflammation and Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells. Comput Math Methods Med 2022:4634925 (2022). PubMed: 35509853
- Chen Y et al. Osteopontin enhances sperm capacitation and in vitro fertilization efficiency in boars. J Anim Sci Technol 64:235-246 (2022). PubMed: 35530410
- Maity J et al. Ferutinin induces osteoblast differentiation of DPSCs via induction of KLF2 and autophagy/mitophagy. Cell Death Dis 13:452 (2022). PubMed: 35552354
- Peindl M et al. EMT, Stemness, and Drug Resistance in Biological Context: A 3D Tumor Tissue/In Silico Platform for Analysis of Combinatorial Treatment in NSCLC with Aggressive KRAS-Biomarker Signatures. Cancers (Basel) 14:N/A (2022). PubMed: 35565305