概述

  • 产品名称Anti-Osteopontin抗体
    参阅全部 Osteopontin 一抗
  • 描述
    山羊多克隆抗体to Osteopontin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    DPKSKEEDKHLK

    , corresponding to C terminal amino acids 288-299 of Human Osteopontin

  • 阳性对照
    • Human thymus, tonsil and spleen lysate. Jurkat and MOLT4 lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab36125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

Blocking peptide available ab45753.

靶标

  • 功能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 组织特异性Bone. Found in plasma.
  • 序列相似性Belongs to the osteopontin family.
  • 翻译后修饰Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody 图像

  • Anti-Osteopontin antibody (ab36125) at 0.3 µg/ml + Human Thymus lysate (RIPA buffer, 30µg total protein per lane).

    Predicted band size : 34 kDa
  • Anti-Osteopontin antibody (ab36125) at 1 µg/ml + lysate prepared from MOLT4 cells (RIPA buffer) at 30 µg

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Ab36125 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

    A) Staining in the germinal centre and interfollicular region.

    B) Positive cells in intraepithelial region.

Anti-Osteopontin antibody (ab36125)参考文献

This product has been referenced in:
  • Wang Y  et al. Peptide nanofibers preconditioned with stem cell secretome are renoprotective. J Am Soc Nephrol 22:704-17 (2011). WB ; Mouse . Read more (PubMed: 21415151) »
  • Chen KC  et al. Evaluation of subchronic toxicity of pet food contaminated with melamine and cyanuric acid in rats. Toxicol Pathol 37:959-68 (2009). Read more (PubMed: 19801503) »

See all 2 Publications for this product

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