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Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.|
|Neutralising||Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 17224478|