概述

  • 产品名称Anti-Osteopontin抗体
    参阅全部 Osteopontin 一抗
  • 描述
    山羊多克隆抗体to Osteopontin
  • 经测试应用适用于: Blocking, ICC/IF, WB, Neutralising, Indirect ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Mouse) expressed in NSO cells.

  • 阳性对照
    • Mouse osteopontin expressed in NSO cells.

性能

应用

Our Abpromise guarantee covers the use of ab11503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.
ICC/IF 1/50.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.
Neutralising Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent dilution. PubMed: 17224478

靶标

  • 功能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 组织特异性Bone. Found in plasma.
  • 序列相似性Belongs to the osteopontin family.
  • 翻译后修饰Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody (ab11503)参考文献

This product has been referenced in:
  • Sharon Y  et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res 75:963-73 (2015). IHC-P ; Mouse . Read more (PubMed: 25600648) »
  • Deangelis T  et al. Growth of V-SRC-transformed cells in serum-free medium through the induction of growth factors. J Cell Physiol : (2012). Read more (PubMed: 23254450) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. ab11503 Osteopontin antibody has only been tested by ELISA, Neutralisation and western blotting. That is not to say that it will not work by immunhistochemistry; just that it is yet to be tested. Searching our on lin...

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The immunogen of antibody is from amino acid 170-183 of human Osteopontin http://www.uniprot.org/uniprot/P10451. The antibody should bind to full length protein as well the immunogen sequence containing fragment.

Thank you for contacting us.
The Isotype control ab37373 can be used with ab11503.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Both of these antibodies are IgG antibodies. Unfortunately, we have not determined the subclass(es) of IgG present but I would expect there to be a mix of subclasses with possibly one predominating but this could vary depending on the immune response o...

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I have received additional information from the lab regarding the procedure that was used.

1) How long should one incubate with the protein (for coating the wells)?
Answer: We coat the recombinant mou...

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The lab sent me the following experimental details for neutralization:

Neutralization: this antibody has the ability to neutralize the bioactivity of recombinant mouse osteopontin. Immobilized recombina...

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The testing discount codehas been activated when your Abreview was submitted. You can now use this code for ordering an Abcamprimary antibody of your choice.
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I am sorry you are experiencing problems to submit an Abreview. As you are using a cell model I have just chosen the option “cell” from Sample type, so the system allows you to continue completing the ...

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I am very pleased to hear you would like to accept our offer and test ab11503 in Rat species with Neutralising assays. This code will give you 1 free primary antibody before the e...

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