Anti-Osteopontin抗体[53] (ab69498)

概述

  • 产品名称
    Anti-Osteopontin抗体[53]
    参阅全部 Osteopontin 一抗
  • 描述
    小鼠单克隆抗体[53] to Osteopontin
  • 特异性
    Recognizes full length human Osteopontin. Does not react with mouse or bovine Osteopontin.
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human Osteopontin

  • 阳性对照
    • Human breast cancer

性能

应用

Our Abpromise guarantee covers the use of ab69498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20688384
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 33 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml.

靶标

  • 功能
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 组织特异性
    Bone. Found in plasma.
  • 序列相似性
    Belongs to the osteopontin family.
  • 翻译后修饰
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

图片

  • All lanes : Anti-Osteopontin antibody [53] (ab69498) at 5 µg/ml

    Lane 1 : MDA-MB-435 cell lysate at 40 µg
    Lane 2 : Conditioned medium from MDA-MB-435 cells containing secreted Osteopontin at 3.8 µg
    Lane 3 : Conditioned medium from MDA-MB-435 cells containing recombinant protein at 0.05 µg

    Secondary
    HRP conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 33 kDa
  • ab69498, at a 2µg/ml, staining human Osteopontin in Breast cancer, by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
  • ab69498 staining Osteopontin in Human mesenchymal stem cell stromas from bone marrow by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with 4% paraformaldehyde and incubated with primary antibody. A Cy3®-conjugated rabbit anti-mouse IgG was used as the secondary antibody.
  • ab69498 staining Osteopontin in Human cancellous bone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.25% Casein for 10 minutes at 23°C. Samples were incubated with primary antibody (1/50 in 10% human serum in TBS) for 1 hour at 23°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG2a polyclonal (1/500) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Li W  et al. Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling. Int J Mol Med 37:63-73 (2016). WB . Read more (PubMed: 26531757) »
  • Felli N  et al. AP2a controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene 35:3016-26 (2016). WB . Read more (PubMed: 26434590) »

See all 10 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Cancellous bone)
Permeabilization
No
Specification
Cancellous bone
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 23°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Sep 26 2016

Thank you for your enquiry.

We do not have set expiration dates for our products.

Most un-conjugated antibodies are stable and can last for several years if stored properly, so we strongly recommend that you follow the storage inst...

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Thank you for contacting us. Abcam recommends heat mediated antigen retrieval using Tris/EDTA pH 9.0 buffer. I have added a link below to our antigen retrieval guide which includes buffer recipes and further information. http://www.abcam.com/inde...

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Thank you for contacting us.
We have many antibodies that specifically stain endothelial and osteoblast marker proteins. The most popular antibodies are as follows;
Osteocalcin, DMP-1 and Osteopontin are Osteoblast marker proteins, the antibo...

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Thank you for contacting us.

I found a reference that appears, from the abstract, to describe characterization of this anti-osteopontin clone, Mab53, but I do not have access to the journal to confirm.

Mapping of functional epitop...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Specification
Bone
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast (MCF-7))
Loading amount
1 µg
Specification
Breast (MCF-7)
Gel Running Conditions
Reduced Denaturing (12% GLYCINE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Prof. Paul Townsend

Verified customer

提交于 Jul 01 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (Renal and Breast)
Loading amount
8 µg
Specification
Renal and Breast
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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