Anti-Osteocalcin抗体[OCG3] (ab13420)

概述

  • 产品名称
    Anti-Osteocalcin抗体[OCG3]
    参阅全部 Osteocalcin 一抗
  • 描述
    小鼠单克隆抗体[OCG3] to Osteocalcin
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • 表位
    Residues 21-31
  • 阳性对照
    • Normal bovine bone tissue
  • 常规说明

    Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.

     

    Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.

性能

应用

Our Abpromise guarantee covers the use of ab13420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.

Use under reducing or non-reducing conditions.

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.

For use in sandwich ELISA with ab13421

ICC/IF Use at an assay dependent concentration. PubMed: 21396708

靶标

  • 功能
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

图片

  • Detection of human osteocalcin by ab13420
  • Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264

文献

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . Read more (PubMed: 28190929) »
  • Jayash SN  et al. Local application of osteoprotegerin-chitosan gel in critical-sized defects in a rabbit model. PeerJ 5:e3513 (2017). IHC ; Rabbit . Read more (PubMed: 28674665) »

See all 34 Publications for this product

客户评价及客户问答

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Application
Immunocytochemistry
Sample
Human Cultured Cells (PULP)
Permeabilization
No
Specification
PULP
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

提交于 Nov 30 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Endochondral bone)
Specification
Endochondral bone
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Sarcoma NOS, ossifying region)
Specification
Sarcoma NOS, ossifying region
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: protease, RT, 5min.
Permeabilization
No
Blocking step
Commercially available blocking agent as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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