Anti-Osteocalcin抗体[OCG3] (ab13420)


  • 产品名称Anti-Osteocalcin抗体[OCG3]
    参阅全部 Osteocalcin 一抗
  • 描述
    小鼠单克隆抗体[OCG3] to Osteocalcin
  • 经测试应用适用于: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • 表位Residues 21-31
  • 阳性对照
    • Normal bovine bone tissue
  • 常规说明

    Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.


    Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.



Our Abpromise guarantee covers the use of ab13420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.

Use under reducing or non-reducing conditions.

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.

For use in sandwich ELISA with ab13421

ICC/IF Use at an assay dependent concentration. PubMed: 21396708


  • 功能Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody [OCG3] 图像

  • Detection of human osteocalcin by ab13420
  • Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264

Anti-Osteocalcin antibody [OCG3] (ab13420)参考文献

This product has been referenced in:
  • Wehrle-Martinez AS  et al. Osteocalcin and Osteonectin Expression in Canine Osteosarcoma. Vet Pathol 53:781-7 (2016). Read more (PubMed: 26926085) »
  • Ravindran S  et al. Biomimetically enhanced demineralized bone matrix for bone regenerative applications. Front Physiol 6:292 (2015). IHC ; Mouse . Read more (PubMed: 26557093) »

See all 17 Publications for this product

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Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

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The osteocalcin antibody ab13420 is provided at a concentration of 2 mg/mL, and we recommend using the antibody at 10 ug/mL in IHC-P. So, the antibody will need to be diluted 1:200 bef...

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Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab13420 in [GUNIEA PIG]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before...

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Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

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Je suis désolé d'apprendre que vous rencontrez toujours des problèmes avec l'anticorps anti-osteocalcin ab13421 que je vous ai envoyé en remplacement du ab...

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