Anti-Osteocalcin抗体[OC4-30] (ab13418)

概述

  • 产品名称Anti-Osteocalcin抗体[OC4-30]
    参阅全部 Osteocalcin 一抗
  • 描述
    小鼠单克隆抗体[OC4-30] to Osteocalcin
  • 经测试应用适用于: WB, IHC-Fr, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • 表位Includes the gamma-carboxylated residue at position 17.

性能

应用

Our Abpromise guarantee covers the use of ab13418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12.0 kDa.
IHC-Fr Use a concentration of 10 µg/ml.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20537381
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody [OC4-30] 图像

  • Immunofluorescence analysis of 1 month osteoblastic-differentiated Human dental pulp stem cells, staining Osteocalcin with ab13418 at 1/80 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
  • ab13418 staining Osteocalcin in Human bone marrow stem cells tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was blocked with 5%BSA + TBS  + 0,5%Tween for 10 minutes. Samples were incubated with primary antibody (1/80) for 14 hours. An undiluted Biotin-conjugated Donkey polyclonal was used as the secondary antibody.

    See Abreview

Anti-Osteocalcin antibody [OC4-30] (ab13418)参考文献

This product has been referenced in:
  • Peng KT  et al. Treatment of critically sized femoral defects with recombinant BMP-2 delivered by a modified mPEG-PLGA biodegradable thermosensitive hydrogel. BMC Musculoskelet Disord 17:286 (2016). WB ; Rabbit . Read more (PubMed: 27421654) »
  • Lu H  et al. Hepcidin promotes osteogenic differentiation through the bone morphogenetic protein 2/small mothers against decapentaplegic and mitogen-activated protein kinase/P38 signaling pathways in mesenchymal stem cells. Mol Med Rep 11:143-50 (2015). WB ; Rat . Read more (PubMed: 25351366) »

See all 17 Publications for this product

Product Wall



Unfortunately, none of the antibodies requested have been tested in sandwich ELISA. They are validated for either direct or indirect ELISA, but they have not been paired to another antibody to work together in the application.

Howev...

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Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nue...

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Thank you for contacting us.

Please buy ab7097.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Once the incubation with secondary antibody is finished then you need to add tertiary antibody which is in your case would be anti rat IgG-HRP. Please incubate for 1 hour at room temperature. Please make sure the t...

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Thank you for contacting us.

We have a Trypsin Enzymatic Antigen Retrieval Solution in our catalogue with reference ab970 (http://www.abcam.com/ab970).

I hope this information is helpful to you. Please do not hesitate to contact u...

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The protocol seems fine to me. However the following updates can be tried

- 0.3% H2O2 can be incubated for 10-15 minutes only.
- The endogenous peroxidases can be blocked after addition of primary antibody. Please check whether the tissue ...

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Thank you for contacting us.

I have asked few questions in my previous email so please send be answers asap. It would be very helpful if you could send me your step by step protocol as I suspect the protocol might needs updates.

Looki...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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This is correct.
Please note that some antibodies work in both IHC-P and IHC-GMA, some do work in IHC-P or IHC-GMA only.
I hope this helps.

Yes we have a recommended protocol for resin (GMA) embedding.
The link to the protocol is: http://www.abcam.com/index.html?pageconfig=resource&rid=11464.
Note that you don't need the antigen retrieval step in your IHC protocol when working ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"