Anti-Osteocalcin抗体(ab76690)

概述

  • 产品名称Anti-Osteocalcin抗体
    参阅全部 Osteocalcin 一抗
  • 描述
    兔多克隆抗体to Osteocalcin
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Osteocalcin aa 2-32 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 632

  • 阳性对照
    • Human cancer tissue (hepatocarcinoma).

性能

应用

Our Abpromise guarantee covers the use of ab76690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 1000 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/1000.

靶标

  • 功能Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody 图像

  • Anti-Osteocalcin antibody (ab76690) at 1/2000 dilution + MG-63 at 20 µg

    Secondary
    Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 11 kDa
  • All lanes : Anti-Osteocalcin antibody (ab76690)

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : Transfected BGLAP

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 11 kDa
  • Formalin fixed, paraffin embedded human cancer tissue (hepatocarcinoma) stained with ab76690 at a 1/50 dilution, followed by treatment with a peroxidase conjugated secondary antibody and DAB staining.

Anti-Osteocalcin antibody (ab76690)参考文献

ab76690 has not yet been referenced specifically in any publications.

Product Wall

Sorry about that.

Here is a new code that is good for testing it in Mouse samples: *, exp 13th Nov 2012.

You can disregard the old code, as it will not be valid.

Apologies again for the mistake.

I hope the experiment...

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DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab76690 in rat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please subm...

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Thank you for your patience.

I have received the following information about the other 3 osteocalcin antibodies I refered to in my last email:


ab13418, ab13421: These antibodies were derived from mice that were immunized with ...

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Thank you for your phone call.

I have checked our osteocalcin antibodies and found one where I know for sure that it was raised against the mature human protein without any modification: ab35078
http://www.abcam.com/Osteocalcin-antibody-...

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Thank you for contacting us. The N terminal osteocalcin protein sequence of rabbit does not have similarity with Rat so the antibodies against the N-terminal end of human or rat Osteocalcin will be suitable and presumably will not react with rabbit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"