概述

  • 产品名称Anti-OSR1抗体
    参阅全部 OSR1 一抗
  • 描述
    兔多克隆抗体to OSR1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse OSR1.

    (Peptide available as ab75079.)

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat Colon Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab74003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

靶标

  • 相关性The OSR1 (odd skipped related 1) gene encodes a zinc-finger transcription factor that is expressed in the germ ring mesendoderm and subsequently in the endoderm and intermediate mesoderm, prior to the expression of definitive kidney or blood/vascular markers. Osr1 expression marks a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney. Osr1 expression is regulated by Runx2 and Ikzf1, which are known as master-gene of osteogenesis and hematopoiesis, respectively.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ODD antibody
    • Odd skipped Drosophila homolog antibody
    • Odd skipped homolog antibody
    • Odd skipped related 1 (Drosophila) antibody
    • Odd skipped related 1 antibody
    • Odd skipped related 1 Drosophila antibody
    • Odd1 antibody
    • Protein odd skipped related 1 antibody
    see all

Anti-OSR1 antibody 图像

  • All lanes : Anti-OSR1 antibody (ab74003) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Colon (Mouse) Tissue Lysate
    Lane 3 : Colon (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 60 kDa,70-75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC-P image of OSR1 antibody (ab74003) on Prostate sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C.

    See Abreview

Anti-OSR1 antibody (ab74003)参考文献

This product has been referenced in:
  • Otani K  et al. Odd-skipped related 1 is a novel tumour suppressor gene and a potential prognostic biomarker in gastric cancer. J Pathol 234:302-15 (2014). IHC ; Human . Read more (PubMed: 24931004) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human fetal kidney cells)
Permeabilization Yes - triton 0.5% in PBS
Specification human fetal kidney cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

提交于 Oct 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"