The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 32 kDa.
OspB, is one of the major Outer Surface Proteins of the outer membrane
of Borrelia burgdorferi, which is composed of various unique outer surface
proteins (Osp) that have been characterized (OspA through OspF). The
osp proteins are lipoproteins anchored by N-terminally attached fatty acid
molecules to the membrane. They are presumed to play a role in
virulence, transmission, or survival in the tick. Two of the major surface
Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins,
show a high degree of sequence similarity, are encoded by a 49-kb
plasmid and share a common promoter, and are coordinately transcribed.
Cell outer membrane; Lipid-anchor.
Outer surface protein B antibody
Western blot - Anti-OspB antibody (ab106082)
All lanes : Anti-OspB antibody (ab106082) at 1/1000 dilution
Lane 1 : MBP-OspB fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary Goat-anti-Rabbit HRP at 1/40000 dilution