Recombinant Protein Epitope Signature Tag, corresponding to amino acids 55 - 185 of Human OSGEPL1.
- Human kidney tissue
RT-4 and U-251 MG lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.
Belongs to the KAE1 / YgjD family.
Information by UniProt
- O sialoglycoprotein endopeptidase like 1 antibody
- O sialoglycoprotein endopeptidase like protein 1 antibody
- osgepl1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-OSGEPL1 antibody (ab122125)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab122125 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-OSGEPL1 antibody (ab122125)
All lanes : Anti-OSGEPL1 antibody (ab122125) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSGEPL1 antibody (ab122125)
ab122125, at a 1/15 dilution, staining OSGEPL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"