Mouse, Xenopus laevis, Cynomolgus monkey
, corresponding to Internal sequence amino acids 715-728 of Human OSBPL10
- Human duodenum and Human testes lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Detection limit dilution -1/32000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the OSBP family.
Contains 1 PH domain.
Information by UniProt
- FLJ20363 antibody
- ORP-10 antibody
- ORP10 antibody
Western blot - Anti-OSBPL10 antibody (ab40824)
Anti-OSBPL10 antibody (ab40824) at 1 µg/ml + Human Duodenum lysate -35µg protein in RIPA bufferSecondary
anti-goat-HRP at 1/3000 dilutionPredicted band size:
84 kDaObserved band size:
90 kDa (why is the actual band size different from the predicted?
)Additional bands at:
55 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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