The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution >1/16000
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 89.4 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
Belongs to the OSBP family. Contains 1 PH domain.
The PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding. The second coiled-coil domain is required for interaction with the tyrosine phosphatase.
Cytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.