1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
应用说明Is unsuitable for Flow Cyt.
功能Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
组织特异性Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
序列相似性Belongs to the OS-9 family. Contains 1 PRKCSH domain.
翻译后修饰Intramolecular disulfide bonds. Isoform 1 and isoform 2 are N-glycosylated.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.
Western blot - OS9 antibody [EPR4272(2)] (ab109510)
All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution
Lane 1 : NIH3T3 cell lysate Lane 2 : 293T cell lysate Lane 3 : HL-60 cell lysate Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 76 kDa
Anti-OS9 antibody [EPR4272(2)] (ab109510)参考文献
This product has been referenced in:
Sun S et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A111:E582-91 (2014).
Read more (PubMed: 24453213) »