概述

  • 产品名称
  • 描述
    兔多克隆抗体to OS9
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human OS9.

    (Peptide available as ab23317.)

  • 阳性对照
    • WB: HeLa, HEK293 and NIH3T3 whole cell lysates and mouse heart, spleen, and liver tissue lysates. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab19853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
  • 组织特异性
    Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
  • 序列相似性
    Belongs to the OS-9 family.
    Contains 1 PRKCSH domain.
  • 翻译后修饰
    Intramolecular disulfide bonds.
    Isoform 1 and isoform 2 are N-glycosylated.
  • 细胞定位
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amplified in osteosarcoma 9 antibody
    • amplified in osteosarcoma antibody
    • ERLEC2 antibody
    • OS-9 antibody
    • Os9 antibody
    • OS9_HUMAN antibody
    • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
    • Protein OS-9 antibody
    see all

图片

  • IHC image of OS9 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19853, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-OS9 antibody (ab19853) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 30 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab19853 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19853, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Erzurumlu Y & Ballar P Androgen Mediated Regulation of Endoplasmic Reticulum-Associated Degradation and its Effects on Prostate Cancer. Sci Rep 7:40719 (2017). Read more (PubMed: 28091582) »
  • Iwamoto S  et al. Reconstructed glycan profile for evaluation of operating status of the endoplasmic reticulum glycoprotein quality control. Glycobiology 23:121-31 (2013). Read more (PubMed: 22975980) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (cerebral cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization
No
Specification
cerebral cortex
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 14 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeP3B)
Total protein in input
6e+006 cells
Specification
HeP3B
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Dec 22 2010

Abreviews
Application
Western blot
Sample
Dog Tissue lysate - other (pancreatic microsomes)
Loading amount
10 µg
Specification
pancreatic microsomes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Felicity Alcock

Verified customer

提交于 Feb 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung, Liver)
Loading amount
25 µg
Specification
Lung, Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells transfected with OS9 tv2-HA)
Specification
HeLa cells transfected with OS9 tv2-HA
Fixative
Methanol
Username

Dr. Felicity Alcock

Verified customer

提交于 Feb 05 2008

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (Rat liver membranes)
Loading amount
10 µg
Specification
Rat liver membranes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Felicity Alcock

Verified customer

提交于 Jan 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells transfected with OS9 tv2-HA)
Total protein in input
600000 cells
Specification
HeLa cells transfected with OS9 tv2-HA
Treatment
S35-labelled
Immuno-precipitation step
Protein A
Username

Dr. Felicity Alcock

Verified customer

提交于 Jan 24 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100000 cells
Specification
HeLa
Treatment
lane 1 - OS9 siRNA lane 2 - laminA siRNA lane 3 - no treatment lane 4 - transfected with OS9 tv2
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Dr. Felicity Alcock

Verified customer

提交于 Jan 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 cells)
Loading amount
10 µg
Specification
HT1080 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Xiaoli Shi

Verified customer

提交于 Nov 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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