为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 180 and 433 of Human Ornithine Decarboxylase (NP_002530).
Our Abpromise guarantee covers the use of ab97395 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
ICC/IF image of ab97395 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97395, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.