兔多克隆抗体to Ornithine Carbamoyltransferase
Synthetic peptide corresponding to Human Ornithine Carbamoyltransferase aa 71-98 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 5009
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab91418 is purified through a protein A column, followed by peptide affinity purification.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/10 - 1/50.
ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Predicted molecular weight: 40 kDa.
Mainly expressed in liver and intestinal mucosa.
Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
Ornithine carbamoyltransferase deficiency
Belongs to the ATCase/OTCase family.
Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
Information by UniProt
EC 220.127.116.11 antibody
Western blot - Ornithine Carbamoyltransferase antibody (ab91418)
Anti-Ornithine Carbamoyltransferase antibody (ab91418) at 1/100 dilution + HepG2 cell line lysate at 35 µg
Predicted band size : 40 kDa
Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab91418)
All lanes : Anti-Ornithine Carbamoyltransferase antibody (ab91418) Lane 1 : SW620 cell lysate Lane 2 : Mouse liver tissue lysate Lane 3 : Rat liver tissue lysate Lysates/proteins at 35 µg per lane. Predicted band size : 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase antibody (ab91418)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human hepatoarcinoma with ab91418, followed by HRP-conjugated secondary antibody and DAB staining.
Flow Cytometry - Anti-Ornithine Carbamoyltransferase antibody (ab91418)
Flow cytometric analysis of ATDC5 cells using ab91418 (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
has not yet been referenced specifically in any publications.
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