Anti-Ornithine Carbamoyltransferase抗体(ab55914)


  • 产品名称Anti-Ornithine Carbamoyltransferase抗体
    参阅全部 Ornithine Carbamoyltransferase 一抗
  • 描述
    兔多克隆抗体to Ornithine Carbamoyltransferase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: LKGRDLLTLK NFTGEEIKYM LWLSADLKFR IKQKGEYLPL LQGKSLGMIF, corresponding to N terminal amino acids 37-86 of Human Ornithine Carbamoyltransferase

  • 阳性对照
    • HepG2 cell lysate Human intestine: epithelial cells of intestinal villus



Our Abpromise guarantee covers the use of ab55914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


Anti-Ornithine Carbamoyltransferase antibody 图像

  • Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Intestine tissue labelling Ornithine Carbamoyltransferase with ab55914 at 8µg/ml. Positively stained epithelial cells of intestinal villus are indicated with arrows. Magnification: 400X

  • All lanes : Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 40 kDa

Anti-Ornithine Carbamoyltransferase antibody (ab55914)参考文献

This product has been referenced in:
  • van Straten G  et al. Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts. PLoS One 9:e100077 (2014). IHC-P ; Dog . Read more (PubMed: 24945279) »

See 1 Publication for this product

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I am very pleased to hear the customer would like to make use of our testing discount offer and test ab55914 in ICC. I can confirm this antibody has not been previously tested (and guaranteed) in this application....

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Thank you for contacting us.
I am sorry but I have as yet not recieved an update as to the restest of the antibody using a different sample (not HepG2). It may be that the tests have not been performed just yet but I will get in contact with the l...

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Thank you for contacting us. That is indeed information that we would be interested in receiving. The antibody ab55914 has as yet not been tested in ICC/IF and if your customer would be willing to submit an Abreview for this that would be great. I h...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Cell (liver)
Specification liver
Fixative Formalin
Permeabilization Yes - Triton
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

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提交于 Dec 22 2010