Anti-ornithine aminotransferase抗体[AT23A2] (ab100845)


  • 产品名称
    Anti-ornithine aminotransferase抗体[AT23A2]
    参阅全部 ornithine aminotransferase 一抗
  • 描述
    小鼠单克隆抗体[AT23A2] to ornithine aminotransferase
  • 宿主
  • 经测试应用
    适用于: IHC-P, WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment purified from E. coli, corresponding to amino acids 33-439 of Human ornithine aminotransferase (NP_000265)

  • 阳性对照
    • HeLa cells and Mouse Liver lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.



Our Abpromise guarantee covers the use of ab100845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • 相关性
    Ornithine aminotransferase (OAT) is a key mitochondrial enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. OAT has also been linked with prostate cancer and alternatively activated macrophages
  • 细胞定位
    Mitochondrion matrix.
  • 数据库链接
  • 别名
    • GACR antibody
    • Gyrate atrophy antibody
    • HOGA antibody
    • OAT antibody
    • OATASE antibody
    • OKT antibody
    • Ornithine aminotransferase (gyrate atrophy) antibody
    • Ornithine aminotransferase mitochondrial antibody
    • ornithine aminotransferase precursor antibody
    • Ornithine aminotransferase, mitochondrial precursor antibody
    • Ornithine delta aminotransferase antibody
    • Ornithine oxo acid aminotransferase antibody
    see all


  • Overlay histogram showing HepG2 cells stained with ab100845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab100845, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • IHC image of ornithine aminotransferase staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100845, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ornithine aminotransferase antibody [AT23A2] (ab100845) at 1/1000 dilution + Mouse Liver Tissue Lysate at 35 µg

    goat anti-mouse conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 49 kDa

  • Immunofluorescence of Human HeLa cells stained with ab100845 (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).


ab100845 has not yet been referenced specifically in any publications.


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