Anti-ornithine aminotransferase抗体(ab137679)
Key features and details
- Rabbit polyclonal to ornithine aminotransferase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ornithine aminotransferase抗体
参阅全部 ornithine aminotransferase 一抗 -
描述
兔多克隆抗体to ornithine aminotransferase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 30-395 of Human ornithine aminotransferase (Uniprot ID: P04181).
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阳性对照
- 293T, A431, HepG2, MOLT4, Raji, Mouse brain whole cell lysates; Human gastric carcinoma tissue; A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137679于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
ICC/IF
1/100 - 1/1000. |
靶标
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相关性
Ornithine aminotransferase (OAT) is a key mitochondrial enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. OAT has also been linked with prostate cancer and alternatively activated macrophages -
细胞定位
Mitochondrion matrix. -
数据库链接
- Entrez Gene: 426430 Chicken
- Entrez Gene: 505323 Cow
- Entrez Gene: 4942 Human
- Entrez Gene: 18242 Mouse
- Entrez Gene: 64313 Rat
- Omim: 613349 Human
- SwissProt: Q3ZCF5 Cow
- SwissProt: P04181 Human
see all -
别名
- GACR antibody
- Gyrate atrophy antibody
- HOGA antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling ornithine aminotransferase with ab137679 at 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed A431 cells labelling ornithine aminotransferase with ab137679 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Anti-ornithine aminotransferase antibody (ab137679) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 49 kDa
7.5% SDS PAGE -
Anti-ornithine aminotransferase antibody (ab137679) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 49 kDa
10% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab137679 被引用在 8 文献中.
- Villar VH et al. Hepatic glutamine synthetase controls N5-methylglutamine in homeostasis and cancer. Nat Chem Biol 19:292-300 (2023). PubMed: 36280791
- Hofsetz E et al. The Mouse Heart Mitochondria N Terminome Provides Insights into ClpXP-Mediated Proteolysis. Mol Cell Proteomics 19:1330-1345 (2020). PubMed: 32467259
- Just PA et al. Lkb1 suppresses amino acid-driven gluconeogenesis in the liver. Nat Commun 11:6127 (2020). PubMed: 33257663
- Soria LR et al. Hepatic glutamine synthetase augmentation enhances ammonia detoxification. J Inherit Metab Dis 42:1128-1135 (2019). PubMed: 30724386
- Robinson JL et al. Prematurity reduces citrulline-arginine-nitric oxide production and precedes the onset of necrotizing enterocolitis in piglets. Am J Physiol Gastrointest Liver Physiol 315:G638-G649 (2018). PubMed: 30048597
- McBrayer SK et al. Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. Cell 175:101-116.e25 (2018). PubMed: 30220459
- Marini JC et al. The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig. Am J Physiol Endocrinol Metab 313:E233-E242 (2017). PubMed: 28611027
- Kitada K et al. High salt intake reprioritizes osmolyte and energy metabolism for body fluid conservation. J Clin Invest 127:1944-1959 (2017). PubMed: 28414295