概述

  • 产品名称Anti-Orexin Receptor 2抗体
    参阅全部 Orexin Receptor 2 一抗
  • 描述
    小鼠多克隆抗体to Orexin Receptor 2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Pig
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 263/282 of Mouse Orexin Receptor 2. Target sequence used to make the antibody:

    VQRKWKQQQPVSQPRGSGQQS

    .

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 51 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides (PubMed:9491897, PubMed:26950369). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed:9491897, PubMed:26950369).
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 结构域The N-terminal region is required for orexin signaling.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hcr tr2 antibody
    • Hcrt receptor 2 antibody
    • Hcrtr2 antibody
    • Hypocretin (orexin) receptor 2 antibody
    • Hypocretin receptor 2 antibody
    • Hypocretin receptor type 2 antibody
    • Orexin 2 receptor antibody
    • Orexin A/Orexin B receptor antibody
    • Orexin receptor 2 antibody
    • Orexin receptor type 2 antibody
    • Orexin type 2 receptor antibody
    • Ox-2-R antibody
    • Ox2-R antibody
    • Ox2r antibody
    • OX2R_HUMAN antibody
    • Type 2 hypocretin receptor antibody
    • Type 2 orexin receptor antibody
    see all

Anti-Orexin Receptor 2 antibody 图像

  • All lanes : Anti-Orexin Receptor 2 antibody (ab43445) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a His tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (His tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 32.5 kDa (why is the actual band size different from the predicted?)

Anti-Orexin Receptor 2 antibody (ab43445)参考文献

This product has been referenced in:
  • Heydendael W  et al. Orexins/hypocretins act in the posterior paraventricular thalamic nucleus during repeated stress to regulate facilitation to novel stress. Endocrinology 152:4738-52 (2011). WB ; Rat . Read more (PubMed: 21971160) »

See 1 Publication for this product

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