Anti-Orexin Receptor 1抗体(ab68718)


  • 产品名称Anti-Orexin Receptor 1抗体
    参阅全部 Orexin Receptor 1 一抗
  • 描述
    兔多克隆抗体to Orexin Receptor 1
  • 特异性No cross reactivity with other proteins
  • 经测试应用适用于: ICC/IF, WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence from the C-terminus of human Orexin Receptor 1, identical to the related mouse and rat sequence.



Our Abpromise guarantee covers the use of ab68718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 48 kDa.Can be blocked with Orexin Receptor 1 peptide (ab188501).
IHC-P Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 5 µg/ml.


Anti-Orexin Receptor 1 antibody 图像

  • Anti-Orexin Receptor 1 antibody (ab68718) at 2 µg/ml + rat brain tissue lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab68718 staining Orexin Receptor 1 in rat brain tissue by Immunohistochemistry (formalin fixed, paraffin embedded tissue section). Primary antibody used at 2µg/ml.
  • ICC/IF image of ab68718 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68718, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Orexin Receptor 1 was immunoprecipitated using 0.5mg Rat Brain tissue lysate, 5µg of Rabbit polyclonal to Orexin Receptor 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68718.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa; Orexin Receptor 1

Anti-Orexin Receptor 1 antibody (ab68718)参考文献

This product has been referenced in:
  • Joshi D & Singh SK Localization and expression of Orexin A and its receptor in mouse testis during different stages of postnatal development. Gen Comp Endocrinol N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27174745) »
  • Chang X  et al. Orexin-A stimulates 3ß-hydroxysteroid dehydrogenase expression and cortisol production in H295R human adrenocortical cells through the AKT pathway. Int J Mol Med 34:1523-8 (2014). Human . Read more (PubMed: 25319929) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Hypothalamus)
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12%)
Loading amount 20 µg
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Verified customer

提交于 Jul 01 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween-20, pH 6.0
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - 0.1% triton-x, PBS
Fixative Paraformaldehyde

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Verified customer

提交于 Jan 08 2014

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