The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Orexins, also called hypocretins, are the common names given to a pair of highly excitatory neuropeptide hormones that were simultaneously discovered by two groups of reseachers in rat brains.
The two highly related peptides (orexin A and B, or hypocretin 1 and 2) are produced by cleavage of a single precursor protein. Studies suggest that orexin A/hypocretin 1 may be of greater biological importance than orexin B/hypocretin 2. Although these peptides are produced by a very small population of cells in the lateral and posterior hypothalamus, they send projections throughout the brain. The orexins/hypocretins are strongly conserved peptides, found in all major classes of vertebrates. The peptides are thought to have arisen early in vertebrate evolution.