Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in water to 1mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - Orexin A (bovine, human, mouse, rat) (ab120212)
ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature. The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Martynska L et al. Orexin A and its role in the regulation of the hypothalamo-pituitary axes in the rat. Reprod Biol6 Suppl 2:29-35 (2006).
Read more (PubMed: 17220939) »