Orexin A (bovine,人,小鼠,大鼠) (ab120212)

概述

  • 产品名称Orexin A (bovine,人,小鼠,大鼠)
  • 描述
    Neuropeptide involved in feeding and sleep
  • 生物学描述Hypothalamic neuropeptide involved in the regulation of feeding, sleep and wakefulness.
  • 纯度> 99%

性能

    应用

    Our Abpromise guarantee covers the use of ab120212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Orexin A (bovine, human, mouse, rat) 图像

    • ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Orexin A (bovine, human, mouse, rat) (ab120212)参考文献

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