Anti-Orexin A抗体[MM0500-8G22] (ab89886)

概述

性能

应用

Our Abpromise guarantee covers the use of ab89886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50 - 1/100.

靶标

  • 相关性Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
  • 细胞定位Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
  • 数据库链接
  • 别名
    • HCRT antibody
    • HCRT1 antibody
    • HCRT2 antibody
    • hypocretin (orexin) neuropeptide precursor antibody
    • Hypocretin 1 antibody
    • Hypocretin 2 antibody
    • hypocretin antibody
    • NRCLP1 antibody
    • Orexin antibody
    • Orexin A antibody
    • Orexin B antibody
    • Orexin precursor antibody
    • OX antibody
    • PPORX antibody
    • PPOX antibody
    • PREPROOREXIN antibody
    see all

Anti-Orexin A antibody [MM0500-8G22] (ab89886)参考文献

ab89886 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Methanol, 0.3%H2O2 as blocking agent for 20 minute(s) · Concentration: 0.3% · Temperature: 21°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Mouse Tissue sections (brain tissue, hypothalamus)
Specification brain tissue, hypothalamus
Permeabilization Yes - BSA
Fixative Formaldehyde
Username

Mrs. Ingrid Hegeman

Verified customer

提交于 Feb 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"