ORC6L overexpression 293T裂解液(全细胞) (ab94161)

概述

  • 产品名称ORC6L overexpression 293T裂解液(全细胞)
    参阅全部 ORC6L 细胞裂解液
  • 描述
    ORC6L overexpression裂解液
  • 常规说明ab94161 is a 293T cell transfected lysate in which Human ORC6L has been transiently over-expressed using a pCMV-ORC6L plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Disease: Defects in ORC6 are the cause of Meier-Gorlin syndrome type 3 (MGORS3) [MIM:613803]. MGORS3 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. Similarity: Belongs to the ORC6 family. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

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应用

Our Abpromise guarantee covers the use of ab94161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ORC6L overexpression 293T lysate (whole cell) 图像

  • ab94161 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-ORC6L antibody (ab88686) at 1/500 dilution

    Lane 1 : ORC6L overexpression 293T lysate (whole cell) (ab94161)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

ORC6L overexpression 293T lysate (whole cell) (ab94161)参考文献

ab94161 has not yet been referenced specifically in any publications.

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