Anti-ORC6L抗体(ab88686)
Key features and details
- Mouse polyclonal to ORC6L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ORC6L抗体 -
描述
小鼠多克隆抗体to ORC6L -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human ORC6L. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human pancreas tissue lysate. Cell lysate from transfected 293T cells. Human placenta. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
疾病相关
Defects in ORC6 are the cause of Meier-Gorlin syndrome type 3 (MGORS3) [MIM:613803]. MGORS3 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. -
序列相似性
Belongs to the ORC6 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 23594 Human
- Omim: 607213 Human
- SwissProt: Q9Y5N6 Human
- Unigene: 49760 Human
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别名
- ORC 6 antibody
- Orc6 antibody
- ORC6_HUMAN antibody
see all
图片
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Anti-ORC6L antibody (ab88686) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa -
All lanes : Anti-ORC6L antibody (ab88686) at 1 µg/ml
Lane 1 : cell lysate from ORC6L transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ab88686 at 3 µg/ml detecting ORC6L in formalin-fixed paraffin-embedded Human placenta by immunohistochemistry.
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ab88686 at 10 µg/ml detecting ORC6L in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab88686 被引用在 1 文献中.
- Zhang K et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). PubMed: 30305611