概述

  • 产品名称Anti-ORC6L抗体
    参阅全部 ORC6L 一抗
  • 描述
    兔多克隆抗体to ORC6L
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide (Human) mapped to a region between residues 230 and 252

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab80406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
    • 疾病相关Defects in ORC6 are the cause of Meier-Gorlin syndrome type 3 (MGORS3) [MIM:613803]. MGORS3 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.
    • 序列相似性Belongs to the ORC6 family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ORC 6 antibody
      • Orc6 antibody
      • ORC6_HUMAN antibody
      • ORC6L antibody
      • Origin recognition complex subunit 6 antibody
      • origin recognition complex subunit 6 homolog like antibody
      • origin recognition complex subunit 6 homolog like (yeast) antibody
      • origin recognition complex subunit 6 like (yeast) antibody
      see all

    Anti-ORC6L antibody 图像

    • All lanes : Anti-ORC6L antibody (ab80406) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 28 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human ORC6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) with ab80406 used for IP (Lane 1) and control IgG (Lane 2) at 3ug/mg lysate and used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds.

    Anti-ORC6L antibody (ab80406)参考文献

    ab80406 has not yet been referenced specifically in any publications.

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