Anti-ORC4L抗体- ChIP Grade (ab9641)

概述

  • 产品名称Anti-ORC4L抗体- ChIP Grade
    参阅全部 ORC4L 一抗
  • 描述
    山羊多克隆抗体to ORC4L - ChIP Grade
  • 经测试应用适用于: ChIP, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide: DVRQWATSSLSWL, corresponding to C terminal amino acids 424-436 of Human ORC4L.

  • 阳性对照
    • Jurkat and NSO (mouse) cell lysates.
  • 常规说明Official Gene Symbol - ORC4L GenBank Accession Number – NP_002543 LocusLink ID - 5000

性能

应用

Our Abpromise guarantee covers the use of ab9641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ORC4L peptide (ab23156).
ELISA Use at an assay dependent concentration.

靶标

  • 功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • 疾病相关Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2) [MIM:613800]. MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.
  • 序列相似性Belongs to the ORC4 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Origin recognition complex, subunit 4, S. cerevisiae, homolog of antibody
    • FLJ46668 antibody
    • HSORC4 antibody
    • ORC 4 antibody
    • ORC 4L antibody
    • ORC 4P antibody
    • ORC4 antibody
    • ORC4_HUMAN antibody
    • ORC4L antibody
    • ORC4L protein antibody
    • ORC4P antibody
    • Origin recognition complex subunit 4 (yeast homolog) like antibody
    • Origin recognition complex subunit 4 antibody
    • Origin recognition complex subunit 4 like (yeast) antibody
    • Origin recognition complex subunit 4 like antibody
    • origin recognition complex, subunit 4 homolog antibody
    • Origin recognition complex, subunit 4, S. cerevisiae, homolog-like antibody
    see all

Anti-ORC4L antibody - ChIP Grade 图像

  • Anti-ORC4L antibody - ChIP Grade (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg

    Predicted band size : 50 kDa
  • Anti-ORC4L antibody - ChIP Grade (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


  • Predicted band size : 50 kDa
    ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
  • All lanes : Anti-ORC4L antibody - ChIP Grade (ab9641) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-ORC4L antibody - ChIP Grade (ab9641)参考文献

This product has been referenced in:
  • Chan KM & Zhang Z Leucine-rich Repeat and WD Repeat-containing Protein 1 Is Recruited to Pericentric Heterochromatin by Trimethylated Lysine 9 of Histone H3 and Maintains Heterochromatin Silencing. J Biol Chem 287:15024-33 (2012). WB . Read more (PubMed: 22427655) »
  • Ghosh S  et al. Assembly of the Human Origin Recognition Complex Occurs through Independent Nuclear Localization of Its Components. J Biol Chem 286:23831-41 (2011). WB ; Human . Read more (PubMed: 21555516) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody was assigned "ChIP grade" status following its application in the following publication; Todorovic V et al. Human origins of ...

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Thank you for your enquiry. Unfortunately, lot #35325 is no longer in stock. Please rest assured, however, that we guarantee that ab9641 will work as stated on the online datasheet or we will provide a replacement or refund. Please contact us again...

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Thank you for your e-mail. I'm really sorry to hear you are having problems with this antibody and would like to give you a few suggestions. You have not mentioned the amount of protein loaded onto the gel. If it is under 30-40ug this may be too low...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"