产品名称 Anti-ORC3L抗体 参阅全部 ORC3L 一抗
IHC-P, WB more details 不适用于:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within the internal sequence amino acids 325-375 of Human ORC3L (NP_036513.2).
HeLa whole cell lysate and 293T cell lysate
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 Affinity purified using an epitope specific to ORC3L immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
应用说明 Is unsuitable for IP.
功能 Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
序列相似性 Belongs to the ORC3 family.
翻译后修饰 Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Homolog of Latheo antibody
homolog of latheo, Drosophila antibody
Anti-ORC3L antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3L antibody (ab99278)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ORC3L with ab99278 at 1/200 (1µg/mg). Detection: DAB.
Western blot - ORC3L antibody (ab99278)
All lanes : Anti-ORC3L antibody (ab99278) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 82 kDa Exposure time : 30 seconds
Anti-ORC3L antibody (ab99278)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"