概述

  • 产品名称Anti-ORC3L抗体
    参阅全部 ORC3L 一抗
  • 描述
    兔多克隆抗体to ORC3L
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 325-375 of Human ORC3L (NP_036513.2).

  • 阳性对照
    • HeLa whole cell lysate and 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified using an epitope specific to ORC3L immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab99278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
    • 序列相似性Belongs to the ORC3 family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Homolog of Latheo antibody
      • homolog of latheo, Drosophila antibody
      • LAT antibody
      • LATHEO antibody
      • LATHEO, drosophila, homolog of antibody
      • orc3 antibody
      • ORC3_HUMAN antibody
      • ORC3L antibody
      • Origin recognition complex subunit 3 antibody
      • Origin recognition complex subunit 3 honolog antibody
      • Origin Recognition Complex Subunit 3 Isoform 1 antibody
      • Origin recognition complex subunit 3 like antibody
      • Origin recognition complex subunit Latheo antibody
      • Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of antibody
      • Origin recognition complex, subunit 3-like (S. cerevisiae) antibody
      • Origin recognition complex, subunit 3-like (yeast) antibody
      see all

    Anti-ORC3L antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ORC3L with ab99278 at 1/200 (1µg/mg). Detection: DAB.
    • All lanes : Anti-ORC3L antibody (ab99278) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 82 kDa


      Exposure time : 30 seconds

    Anti-ORC3L antibody (ab99278)参考文献

    ab99278 has not yet been referenced specifically in any publications.

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