Customer reviews and FAQs

概述

  • 产品名称
    Anti-ORC2抗体[SB46]
    参阅全部 ORC2 一抗
  • 描述
    小鼠单克隆抗体[SB46] to ORC2
  • 经测试应用
    适用于: IP, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human, Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein (Saccharomyces cerevisiae).

性能

应用

Our Abpromise guarantee covers the use of ab31930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 66 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • 序列相似性
    Belongs to the ORC2 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • orc2 antibody
      • ORC2_HUMAN antibody
      • ORC2L antibody
      • Origin of replication 2 (yeast homolog) like antibody
      • Origin recognition complex protein 2 homolog antibody
      • Origin recognition complex subunit 2 antibody
      • Origin recognition complex subunit 2 isoform B antibody
      • Origin recognition complex subunit 2 like (yeast) antibody
      • Origin recognition complex, subunit 2 homolog antibody
      • Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
      • Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
      see all

    Anti-ORC2 antibody [SB46] 图像

    • Western blot - Anti-ORC2 antibody [SB46] (ab31930)
      Western blot - Anti-ORC2 antibody [SB46] (ab31930)Image from Granata M et al, PLoS Genet. 2010 Aug 5;6(8). pii: e1001047, Fig S1.


      Predicted band size : 66 kDa

      Image from Granata M et al, PLoS Genet. 2010 Aug 5;6(8). pii: e1001047, Fig S1.

      The Western blots in which the presence of Rad9 was analyzed in the total (T), soluble (S) and chromatin-enriched (Ch) fractions from wt (K699) cells, were controlled for proper fractionation of control proteins, known to remain in the soluble fraction (Tubulin) or to bind to chromatin (Orc2, ab31930).
    • Flow Cytometry - Anti-ORC2 antibody [SB46] (ab31930)
      Flow Cytometry - Anti-ORC2 antibody [SB46] (ab31930)
      Overlay histogram showing HeLa cells stained with ab31930 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31930, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-ORC2 antibody [SB46] (ab31930)参考文献

    This product has been referenced in:
    • Hiraga SI  et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). Flow Cyt ; Human . Read more (PubMed: 28077461) »
    • Granata M  et al. Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activity. PLoS Genet 6: (2010). WB ; Saccharomyces cerevisiae . Read more (PubMed: 20700441) »

    See all 2 Publications for this product

    发表研究结果有使用 ab31930？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab31930 的 abreview 提交 ab31930 的问题

    1 Abreview or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ORC2 antibody [SB46]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (Hela cell subcellular fractionation)
    Loading amount
    10 µg
    Specification
    Hela cell subcellular fractionation
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Dec 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册