- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-ORC2抗体[SB46]
参阅全部 ORC2 一抗 -
描述小鼠单克隆抗体[SB46] to ORC2
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宿主Mouse
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经测试应用适用于: IP, WB, Flow Cytmore details
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种属反应性与反应: Human, Saccharomyces cerevisiae
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免疫原
Recombinant full length protein (Saccharomyces cerevisiae).
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.01% Sodium Azide.
Constituents: 50% Glycerol, 1mg/ml BSA. -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号SB46
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骨髓瘤Sp2/0
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab31930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 66 kDa. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
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序列相似性Belongs to the ORC2 family.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 4999 Human
- Omim: 601182 Human
- SwissProt: Q13416 Human
- Unigene: 444870 Human
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别名
- orc2 antibody
- ORC2_HUMAN antibody
- ORC2L antibody
see all
图片
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![Western blot - Anti-ORC2 antibody [SB46] (ab31930)](http://www.abcam.cn/ps/products/31/ab31930/Images/ORC2-Primary-antibodies-ab31930-1.jpg)
Western blot - Anti-ORC2 antibody [SB46] (ab31930)Image from Granata M et al, PLoS Genet. 2010 Aug 5;6(8). pii: e1001047, Fig S1.The Western blots in which the presence of Rad9 was analyzed in the total (T), soluble (S) and chromatin-enriched (Ch) fractions from wt (K699) cells, were controlled for proper fractionation of control proteins, known to remain in the soluble fraction (Tubulin) or to bind to chromatin (Orc2, ab31930). -
![Flow Cytometry - Anti-ORC2 antibody [SB46] (ab31930)](http://www.abcam.cn/ps/products/31/ab31930/Images/ORC2-Primary-antibodies-ab31930-2.jpg)
Flow Cytometry - Anti-ORC2 antibody [SB46] (ab31930)Overlay histogram showing HeLa cells stained with ab31930 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31930, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
文献
This product has been referenced in:
- Roy S et al. p53 orchestrates DNA replication restart homeostasis by suppressing mutagenic RAD52 and POL? pathways. Elife 7:N/A (2018). Read more (PubMed: 29334356) »
- Hernández G et al. Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Nat Commun 9:967 (2018). Read more (PubMed: 29511213) »
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