Anti-ORC2抗体(ab99277)
Key features and details
- Rabbit polyclonal to ORC2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ORC2抗体
参阅全部 ORC2 一抗 -
描述
兔多克隆抗体to ORC2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1-50 (MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII) of Human ORC2 (NP_006181.1).
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阳性对照
- Hela and 293T whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
序列相似性
Belongs to the ORC2 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4999 Human
- Omim: 601182 Human
- SwissProt: Q13416 Human
- Unigene: 444870 Human
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别名
- orc2 antibody
- ORC2_HUMAN antibody
- ORC2L antibody
see all
图片
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All lanes : Anti-ORC2 antibody (ab99277) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ORC2 with ab99277 at 1/1000 (0.2µg/mg). Detection: DAB.
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Detection of ORC2 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab99277 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab99277 被引用在 1 文献中.
- Deakyne JS et al. Structural and Functional Basis for an EBNA1 Hexameric Ring in Epstein-Barr Virus Episome Maintenance. J Virol 91:N/A (2017). PubMed: 28701406