概述

  • 产品名称Anti-ORC2抗体
    参阅全部 ORC2 一抗
  • 描述
    小鼠单克隆抗体to ORC2
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length ORC2 protein (Human), with a tag. MW of the tag alone is 26kDa.

  • 阳性对照
    • Jurkat cell lysate and human spleen tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab68348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21029866
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • 序列相似性Belongs to the ORC2 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • orc2 antibody
    • ORC2_HUMAN antibody
    • ORC2L antibody
    • Origin of replication 2 (yeast homolog) like antibody
    • Origin recognition complex protein 2 homolog antibody
    • Origin recognition complex subunit 2 antibody
    • Origin recognition complex subunit 2 isoform B antibody
    • Origin recognition complex subunit 2 like (yeast) antibody
    • Origin recognition complex, subunit 2 homolog antibody
    • Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
    • Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
    see all

Anti-ORC2 antibody 图像

  • Anti-ORC2 antibody (ab68348) at 1/500 dilution + Jurkat cell lysate at 25 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 66 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase of ab68348 on formalin-fixed paraffin-embedded human spleen tissue, antibody concentration at 5µg/ml.

Anti-ORC2 antibody (ab68348)参考文献

This product has been referenced in:
  • Ramos AC  et al. The kin17 Protein in Murine Melanoma Cells. Int J Mol Sci 16:27912-20 (2015). WB . Read more (PubMed: 26610484) »
  • Qattan AT  et al. Spatial Distribution of Cellular Function: The Partitioning of Proteins between Mitochondria and the Nucleus in MCF7 Breast Cancer Cells. J Proteome Res : (2012). Read more (PubMed: 23051583) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab68348.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"