概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to ORC2
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length ORC2 protein (Human), with a tag. MW of the tag alone is 26kDa.

  • 阳性对照
    • Jurkat cell lysate and human spleen tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab68348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21029866
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • 序列相似性
    Belongs to the ORC2 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • orc2 antibody
    • ORC2_HUMAN antibody
    • ORC2L antibody
    • Origin of replication 2 (yeast homolog) like antibody
    • Origin recognition complex protein 2 homolog antibody
    • Origin recognition complex subunit 2 antibody
    • Origin recognition complex subunit 2 isoform B antibody
    • Origin recognition complex subunit 2 like (yeast) antibody
    • Origin recognition complex, subunit 2 homolog antibody
    • Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
    • Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
    see all

Anti-ORC2 antibody 图像

  • Anti-ORC2 antibody (ab68348) at 1/500 dilution + Jurkat cell lysate at 25 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 66 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase of ab68348 on formalin-fixed paraffin-embedded human spleen tissue, antibody concentration at 5µg/ml.

Anti-ORC2 antibody (ab68348)参考文献

This product has been referenced in:
  • Wu R  et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . Read more (PubMed: 27679476) »
  • Ramos AC  et al. The kin17 Protein in Murine Melanoma Cells. Int J Mol Sci 16:27912-20 (2015). WB . Read more (PubMed: 26610484) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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