概述

  • 产品名称Anti-ORC1抗体
    参阅全部 ORC1 一抗
  • 描述
    兔多克隆抗体to ORC1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 811 and 861 of human ORC1 (NP_004144.2)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85830 was affinity purified using an epitope specific to ORC1 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab85830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • 序列相似性Belongs to the ORC1 family.
    Contains 1 BAH domain.
  • 发展阶段Expression is cell-cycle regulated, it starts to accumulate in mid-G1 phase, reaches a peak at the G1/S boundary, and decreases to a basal level in S phase (at protein level).
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSORC1 antibody
    • MmORC1 antibody
    • orc1 antibody
    • ORC1_HUMAN antibody
    • ORC1L antibody
    • Origin Recognition Complex 1 antibody
    • Origin recognition complex subunit 1 (yeast homolog) like antibody
    • Origin recognition complex subunit 1 antibody
    • Origin recognition complex subunit 1 homolog antibody
    • Origin recognition complex subunit 1 like (S. cerevisia antibody
    • Origin recognition complex subunit 1 like antibody
    • Origin recognition complex subunit 1 S. cerevisiae homolog like antibody
    • Origin recognition complex, subunit 1 like (yeast) antibody
    • PARC1 antibody
    • Replication control protein 1 antibody
    see all

Anti-ORC1 antibody 图像

  • All lanes : Anti-ORC1 antibody (ab85830) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 97 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of Human ORC1 by Western Blot of Immunprecipitate.
    All lanes: ab85830 at 1µg/ml staining ORC1 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
    Lane 1: ab85830
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-ORC1 antibody (ab85830)参考文献

This product has been referenced in:
  • Maya-Mendoza A  et al. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis 5:e1351 (2014). WB ; Human . Read more (PubMed: 25058425) »
  • Chan KM & Zhang Z Leucine-rich Repeat and WD Repeat-containing Protein 1 Is Recruited to Pericentric Heterochromatin by Trimethylated Lysine 9 of Histone H3 and Maintains Heterochromatin Silencing. J Biol Chem 287:15024-33 (2012). WB . Read more (PubMed: 22427655) »

See all 2 Publications for this product

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