Anti-ORC1抗体(ab85830)
Key features and details
- Rabbit polyclonal to ORC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ORC1抗体
参阅全部 ORC1 一抗 -
描述
兔多克隆抗体to ORC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide, corresponding to a region between residues 811 and 861 of human ORC1 (NP_004144.2)
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85830 was affinity purified using an epitope specific to ORC1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85830于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
序列相似性
Belongs to the ORC1 family.
Contains 1 BAH domain. -
发展阶段
Expression is cell-cycle regulated, it starts to accumulate in mid-G1 phase, reaches a peak at the G1/S boundary, and decreases to a basal level in S phase (at protein level). -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4998 Human
- Omim: 601902 Human
- SwissProt: Q13415 Human
- Unigene: 17908 Human
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别名
- HSORC1 antibody
- MmORC1 antibody
- orc1 antibody
see all
图片
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All lanes : Anti-ORC1 antibody (ab85830) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of Human ORC1 by Western Blot of Immunprecipitate.
All lanes: ab85830 at 1µg/ml staining ORC1 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: ab85830
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab85830 被引用在 4 文献中.
- Wang Y et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
- Chen X et al. Up-regulated lncRNA XIST contributes to progression of cervical cancer via regulating miR-140-5p and ORC1. Cancer Cell Int 19:45 (2019). PubMed: 30858762
- Maya-Mendoza A et al. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis 5:e1351 (2014). WB ; Human . PubMed: 25058425
- Chan KM & Zhang Z Leucine-rich Repeat and WD Repeat-containing Protein 1 Is Recruited to Pericentric Heterochromatin by Trimethylated Lysine 9 of Histone H3 and Maintains Heterochromatin Silencing. J Biol Chem 287:15024-33 (2012). WB . PubMed: 22427655