概述

  • 产品名称Anti-Orai1抗体
    参阅全部 Orai1 一抗
  • 描述
    兔多克隆抗体to Orai1
  • 特异性Detects both the glycosylated and un-glycosylated forms of Orai1.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    STTSGSRRSRRRSGDGEPP

    , corresponding to intracellular amino acids 22-40 of Human Orai1

  • 阳性对照
    • Cell membrane preparations containing protease inhibitors. Jurkat whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab78471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明ICC/IF: 1/1000 - 1/2500.
    IP: Use at 1µg.
    WB: 1/1000 - 1/5000. Detects a band of approximately 50 and 35-50 kDa (predicted molecular weight: 33 kDa). By Western blot the antibody reacts with the ~50kDa Orai1 protein. Additional bands between 35 and 50kDa may be seen if analyzing over-expressed Orai1. These additional bands most likely represent different degrees of glycosylation of Orai1.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
    • 疾病相关Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
    • 序列相似性Belongs to the Orai family.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Calcium release activated calcium channel protein 1 antibody
      • Calcium release activated calcium modulator 1 antibody
      • Calcium release-activated calcium channel protein 1 antibody
      • CRACM 1 antibody
      • CRACM1 antibody
      • CRCM1 antibody
      • CRCM1_HUMAN antibody
      • FLJ14466 antibody
      • Orai 1 antibody
      • ORAI calcium release activated calcium modulator 1 antibody
      • orai1 antibody
      • ORAT1 antibody
      • Protein orai 1 antibody
      • Protein orai-1 antibody
      • TMEM 142A antibody
      • TMEM142A antibody
      • Transmembrane protein 142A antibody
      see all

    Anti-Orai1 antibody 图像

    • Lane 1 : Anti-Orai1 antibody (ab78471) at 0.5 µg/ml
      Lane 2 : Anti-Orai1 antibody (ab78471) at 1 µg/ml
      Lane 3 : Anti-Orai1 antibody (ab78471) at 5 µg/ml

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 35-50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    Anti-Orai1 antibody (ab78471)参考文献

    This product has been referenced in:
    • Yang B & Bouchard MJ The hepatitis B virus X protein elevates cytosolic calcium signals by modulating mitochondrial calcium uptake. J Virol 86:313-27 (2012). WB . Read more (PubMed: 22031934) »
    • Khadra N  et al. CD95 triggers Orai1-mediated localized Ca2+ entry, regulates recruitment of protein kinase C (PKC) ß2, and prevents death-inducing signaling complex formation. Proc Natl Acad Sci U S A 108:19072-7 (2011). Read more (PubMed: 22065776) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - other (Jurkat T cells)
    Loading amount 40 µg
    Specification Jurkat T cells
    Gel Running Conditions Reduced Denaturing (10%/5% stacking)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Dr. Barbara Niemeyer

    Verified customer

    提交于 Jun 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"