概述

  • 产品名称Anti-Orai1抗体
    参阅全部 Orai1 一抗
  • 描述
    兔多克隆抗体to Orai1
  • 特异性ab59330 is predicted to have no cross-reactivity to Orai2 or Orai3.
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    An 18 amino acid peptide from near the amino terminus of human Orai1.

  • 阳性对照
    • Human ovary tissue lysate and human spleen tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21632714
Flow Cyt Use at an assay dependent concentration. PubMed: 19965684ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).

靶标

  • 功能Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
  • 疾病相关Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
  • 序列相似性Belongs to the Orai family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium release activated calcium channel protein 1 antibody
    • Calcium release activated calcium modulator 1 antibody
    • Calcium release-activated calcium channel protein 1 antibody
    • CRACM 1 antibody
    • CRACM1 antibody
    • CRCM1 antibody
    • CRCM1_HUMAN antibody
    • FLJ14466 antibody
    • Orai 1 antibody
    • ORAI calcium release activated calcium modulator 1 antibody
    • orai1 antibody
    • ORAT1 antibody
    • Protein orai 1 antibody
    • Protein orai-1 antibody
    • TMEM 142A antibody
    • TMEM142A antibody
    • Transmembrane protein 142A antibody
    see all

Anti-Orai1 antibody 图像

  • All lanes : Anti-Orai1 antibody (ab59330) at 1 µg/ml

    Lane 1 : human ovary tissue lysate with no blocking peptide
    Lane 2 : human ovary tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab59330 at 10ug/ml staining Orai1 in human spleen by Immunohistochemistry.

Anti-Orai1 antibody (ab59330)参考文献

This product has been referenced in:
  • Borst O  et al. The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes. Blood 119:251-61 (2012). WB . Read more (PubMed: 22031864) »
  • Gómez-Fernández C  et al. Calcium signaling in mouse oocyte maturation: the roles of STIM1, ORAI1 and SOCE. Mol Hum Reprod 18:194-203 (2012). Read more (PubMed: 22053056) »

See all 6 Publications for this product

Product Wall

This sequence information is proprietary and unfortunately we cannot disclose it.

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am atta...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"