概述

  • 产品名称Anti-Optineurin抗体
    参阅全部 Optineurin 一抗
  • 描述
    兔多克隆抗体to Optineurin
  • 经测试应用适用于: ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig, Cynomolgus Monkey, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    GEVLPDIDTLQIHVMDC

    , corresponding to amino acids 575-591 of Human Optineurin.

  • 阳性对照
    • Murine or human heart, brain, or fibroblast lysates.

性能

应用

Our Abpromise guarantee covers the use of ab23666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).
ICC Use at an assay dependent concentration.

靶标

  • 功能Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.
  • 组织特异性Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.
  • 疾病相关Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
    Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
    Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • 结构域Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.
  • 翻译后修饰Phosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time.
  • 细胞定位Cytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14.7K interacting protein antibody
    • Ag9 C5 antibody
    • ALS12 antibody
    • E3 14.7K interacting protein antibody
    • E3-14.7K-interacting protein antibody
    • FIP 2 antibody
    • FIP-2 antibody
    • FIP2 antibody
    • Glaucoma 1 open angle E (adult onset) antibody
    • Glaucoma 1 open angle E antibody
    • GLC1E antibody
    • HIP 7 antibody
    • HIP-7 antibody
    • HIP7 antibody
    • Huntingtin interacting protein 7 antibody
    • Huntingtin interacting protein HYPL antibody
    • Huntingtin interacting protein L antibody
    • Huntingtin yeast partner L antibody
    • Huntingtin-interacting protein 7 antibody
    • Huntingtin-interacting protein L antibody
    • HYPL antibody
    • Injury inducible protein I 55 antibody
    • NEMO related protein antibody
    • NEMO-related protein antibody
    • NRP antibody
    • Optic neuropathy inducing protein antibody
    • Optic neuropathy-inducing protein antibody
    • Optineurin antibody
    • OPTN antibody
    • OPTN_HUMAN antibody
    • TFIIIA IntP antibody
    • TFIIIA-IntP antibody
    • Transcription factor IIIA interacting protein antibody
    • Transcription factor IIIA-interacting protein antibody
    • Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains antibody
    see all

Anti-Optineurin antibody 图像

  • ab23666 staining Optineurin in HeLa cells and Retinal Ganglion Cell line 5 cells (RGC-5) by Immunocytochemistry/ Immunofluorescence.

    (A) HeLa cells were pulsed with Alexa-546® conjugated transferrin for 30 minutes, chased with unlabeled holo-transferrin for 20 minutes and stained for endogenous Optineurin.

    (B) RGC-5 cells were stained for endogenous Optineurin.

  • Anti-Optineurin antibody (ab23666) at 1 µg + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa,43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ab23666 staining Optineurin in Mouse cultured primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.25% Triton in PBS prior to blocking in 5% Donkey serum for 30 minutes at 22°C. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at 22°C. The secondary antibody was Cy3®-conjugated Donkey anti-Rabbit polyclonal, diluted 1/500.

    See Abreview

Anti-Optineurin antibody (ab23666)参考文献

This product has been referenced in:
  • Farrawell NE  et al. Distinct partitioning of ALS associated TDP-43, FUS and SOD1 mutants into cellular inclusions. Sci Rep 5:13416 (2015). Read more (PubMed: 26293199) »
  • Meunier E  et al. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature 509:366-70 (2014). Read more (PubMed: 24739961) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step fish gelantine and ovalbumine as blocking agent for 30 minute(s) · Concentration: 0.6% · Temperature: 22°C
Sample Mouse Tissue sections (Retina)
Specification Retina
Permeabilization Yes - PBS-Tween 0.01%
Fixative only frozen
Username

Abcam user community

Verified customer

提交于 Jul 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0,25% Triton in PBS
Blocking step Donkey Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain lysate differentially centrifuged)
Loading amount 5 µg
Specification brain lysate differentially centrifuged
Gel Running Conditions Non-reduced Denaturing (12 % SDS Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"