Optiblot Prestained Marker (ab119210)

概述

  • 产品名称Optiblot Prestained Marker
    参阅全部 Prestained Protein Ladder 试剂
  • 经测试应用适用于: WB, SDS-PAGEmore details
  • 常规说明Optiblot Prestained Markers have been specifically formulated for use with Optiblot Gels and Buffers. Marker proteins are conjugated to dye molecules to show position before staining indicating how far your protein has migrated and to confirm the blotting efficiency without the need to stain your target protein.

    Optiblot Prestained Markers consist of 195, 142, 96, 71, 48, 33, 28, 22, 12 and 7.6 kDa standards. For additional convenience and ease of identification, the 71 and 28 kDa standards have been conjugated to an orange dye molecule.

    A load of only 4 µl Optiblot Pre-stained Markers is sufficient for a high intensity marker ladder (nearly 2.5x less loading than conventional ladders).

    The prestained markers are provided ready to use in 1x Optiblot sample buffer(ab119196). Recommended volumes to load for a 12 well comb are:

    To Monitor during run: 10 µl/well
    Visisble after blotting: 4 µl/well
    Visible after staining: 4 µl/well

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

性能

  • 别名
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder

应用

Our Abpromise guarantee covers the use of ab119210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Optiblot Prestained Marker 图像

  • ab119210 separated on a 12%, 12 well Optiblot gel.

    Lane 1: Prior to staining with Optiblot blue (ab119211)
    Lane 2: After staining with Optiblot blue (ab119211)

Optiblot Prestained Marker (ab119210)参考文献

ab119210 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. My colleague in the lab informed me that our optiblot range of protein molecular weight markers do contain His-tagged proteins. The markers ab115832 and ab48854 do not contain His-tagged proteins. I hope this helps, please ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"