Optiblot ECL Detect试剂盒(23pg-187ng) (ab133406)

概述

  • 产品名称Optiblot ECL Detect试剂盒(23pg-187ng)
    参阅全部 ECL Substrate 试剂盒
  • 产品概述

    Optiblot ECL Detect Kit (ab133406) is an enhanced chemiluminescent substrate specially developed for film imaging and is compatible with CCD imaging. Optiblot ECL Detect Kit produces a strong signal with very low background for high sensitivity. Additionally, the Optiblot ECL signal is long lasting, allowing repeated exposures without fear of losing data.

    200ml kit for 2000cm2 membrane

    500ml kit for 5000cm2 membrane

     

  • 说明

    Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.

     

    Recommended primary antibody dilution range: 1/5000 - 1/20,000

    Recommended secondary antibody dilution range: 1/20,000 - 1/100,000

     

    *Due to sensitivity of substrate, optimisation may be required:

    Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.

     

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab133406 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent concentration.

    Optiblot ECL Detect Kit (23pg-187ng) 图像

    • Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.

       

      Secondary

      HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

    • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.

    • Anti-COX2 antibody (ab15191) at 1/25000 dilution. Lanes 1-14 COX2 protein (ab58868), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.

       

      Secondary:

      HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

    • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.

    实验方案

    Optiblot ECL Detect Kit (23pg-187ng) (ab133406)参考文献

    This product has been referenced in:
    • Watts MN  et al. Decreased retinal blood flow in experimental colitis; improvement by eye drop administration of losartan. Exp Eye Res 115C:22-26 (2013). Read more (PubMed: 23830910) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    The solution is highly sensitive, generating signal very fast (beta actin came in 20-30 sec, using the optiblot ECL detection solution developed in chem doc instrument).
    Username

    Abcam user community

    Verified customer

    提交于 Nov 09 2015

    Visualization of Western blots

    Good Good 4/5 (Ease of Use)
    Abreviews
    This abcam optiblot detect kit gives signal very fast like that of the west dura. For my protein of interest, Signal appeared between 50sec to 1.3mins in chemidoc, B-actin comes even faster (30-40sec). The concentration of the total protein loaded was 40ug).
    Username

    Abcam user community

    Verified customer

    提交于 Aug 07 2015

    ab133409 has been used for ELISA applications.

    However, since there are many different readers, plates, etc., testing/evaluation is necessary to assess utility for your specific experimental conditions.

    Thank you for your enquiry.

    I am sorry to confirm that ab133406 is not suitable for detection of AP. This detection system has been designed for optimal detection of HRP, and will not be as sensitive for detection of AP.

    I am sorr...

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    Thank you for contacting us.

    The alternative we have is ab133406; http://www.abcam.com/Optiblot-ECL-Detect-Kit-23pg-187ng-ab133406.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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    Thank you for contacting us. I hope that you are well.

    The ab133410 is a fluorescence based kit however the Roche kit cat # 11520709001 is a chemiluminescence based so this will not be a right alternative.

    Please suggest ab133406, htt...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"