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3 Abreviews or Q&A
What is the concentration of the Optiblot DTT Reducer (10x) ab119199
Asked on Apr 17 2013
The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...
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回复于 Apr 17 2013
Hi, Regarding the DTT, I actually need them urgently, as I am planning to start immunoblotting from coming Monday. Since, I am currently working on a manuscript, it will be slightly problematic to get them later. Also, can we not store the unused ...
Asked on Oct 02 2012
I now have further information in regards to the storage of the Optiblot DTT Reducer (ab119199). I can confirm that this should be fine andshould remain stable for longer if stored at -20°C. I would recommend dividing the 1 mL into smaller aliquots...
回复于 Oct 02 2012
I tested four samples and one control (HeLa RIPA lysate).
Loading buffer has DTT (100mM final) and SDS (2% final). Samples are boiled.
I guess I was wondering whether you guys have tested the dimerization of MCTP1 (if that's not possible,...
Asked on Aug 09 2012
Thank you for your reply.
I have not seen that we have tested or have any additional data regarding the dimerization of this protein or which reducing agents might be more effective. We generally use a 2x Laemmli buffer with 4% SDS, or use o...
回复于 Aug 09 2012
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