Optiblot Bradford Reagent offers an improved formulation over classical Bradford that cannot tolerate detergent contamination of the protein samples. In addition, the Optiblot Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations. When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443